Essential Estrogens® - Hormone Test

Essential Estrogens® - Hormone Test

The Essential Estrogens® hormone test provides a focused overview of estrogen balance in both pre- and post-menopausal women. Using a single urine sample, the Essential Estrogens® hormone test evaluates the dynamics of sex steroid metabolism that can profoundly affect a woman´s health throughout her lifetime. The Essential Estrogens® hormone test is designed to assist in the prevention and treatment of estrogen-related symptoms and conditions. This robust urine analysis is ideal for prescribing and monitoring Hormone Replacement Therapy (HRT) and for assessing the risk of estrogen-related diseases, including breast cancer risk, prostate disease, osteoporosis, and auto-immune disease.

Female Hormones and Hormone Testing
Hormones are tremendously complex. During the pre-menopausal years, imbalanced hormones can pose challenges with mood stabilization, conception, and regulations of the menstrual cycle.
Many women entering their 40’s and 50’s experience a range of symptoms from hormonal imbalance, including loss of sex drive, “brain fog”, weight gain, hot flashes, irregular menstrual cycles, vaginal dryness, urinary incontinence, depression and/or changes in sleep patterns.

Essential Estrogens® Hormone Testing and Hormone Replacement Therapy (HRT)
From the onset of ones period through menopause and beyond, proper hormone balance affects many of the issues most important to women: fertility, sex, relationships, and even ones outlook on life. Establishing hormonal baseline measures is essential to determining the need for hormone replacement therapy (HRT) or other means of balancing hormones.

The Essential Estrogens® hormone test measures the following urinary estrogens and estrogen metabolites, and includes an assessment of estrogen methylation, which is a critical step in the protective mechanism of estrogen metabolism.


  • Estrone 1 (E1)
  • Estradiol (E2)
  • Estriol (E3)

Estrogen Metabolites:

  • 2-Hydroxy-estrone
  • 2-Methoxy-estrone
  • 4-Hydroxy-estrone
  • 4-Methoxy-estrone
  • 16-Alpha-Hydroxy-estrone

2:16 Ratio:

  • 2-Hydroxy-estrone/16-Alpha-Hydroxy-estrone ratio

Estrogen Methylation:

  • 2-Methoxy-estrone/2-Hydroxy-estrone ratio

Estrogens, particularly estrone and estradiol, are well described in literature regarding the health and disease of estrogen sensitive tissues.

Estrogen deficiency:

  • Osteoporosis
  • Amenorrhea
  • Menopausal symptoms

Estrogen excess:

  • Menorrhagia
  • Uterine Fibroids
  • Anxiety/Irritability
  • Breast Cancer

Estrogen metabolites are proven to convey protection (if balanced) or increased risk (if imbalanced) for disease. Estrogen metabolite imbalance results in increased risk of:

  • Breast Cancer
  • Osteoporosis
  • Prostate Cancer

Estrogen methylation is a critical step in the protective mechanism of estrogen metabolism. Methylation is required for the protective effects of 2-hydroxy-estrone, and for the same detoxification of 4-hydroxy-estrone.

Test Type: Urine Test


  • 16 alpha-hydroxyestrone
  • 2-Hydroxyestrone
  • 2-Methoxyestrone
  • 4-Hydroxyestrone
  • 4-Methoxyestrone
  • Creatinine
  • Estradiol
  • Estriol
  • Estrone


  •  16 alpha-hydroxyestrone82671  
  •  2-Hydroxyestrone82671  
  •  2-Methoxyestrone82671  
  •  4-Hydroxyestrone82671  
  •  4-Methoxyestrone82671  
  •  Estradiol82670  
  •  Estriol82677  
  •  Estrone82679  

Specimen Requirements:  120ml Aliquot of urine (24-hour urine collection) with Aldosterone OR 120ml Aliquot of urine (first morning void)

Turn Around Time: 

21 Days

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This website is intended for educational purposes only. It is not meant for use as diagnostic information and is part of Genova Diagnostics' communication with physicians and patients.