GI Effects Microbial Ecology Stool Profile

When Should the GI Effects Microbial Ecology Profile Be Considered?

Patients with a clinical history suggestive of a gastrointestinal infection or dysbiosis can be evaluated with the GI Effects Microbial Ecology Profile. Symptoms such as gas, bloating, abdominal pain, diarrhea, and constipation may be a result of a microbial imbalance or infection. This profile can also be used to monitor treatment efficacy to eradicate an organism or to monitor changes to the microbiota.

Gut microbes are codependent with one another and with their human host, and the health of one affects the other. A sizeable volume of research associates a dysbiotic, or imbalanced gut microbiome with multiple disease states both within and outside of the GI tract.^1,2 The microbiome's diverse metabolic activities ultimately impact the human host, and the human hosts activities ultimately affect the microbiome's health.

The GI Effects Microbial Ecology Profile biomarkers include:

• Commensal Bacteria demonstrate the gut microbiomes composition and relative abundance.
  • More than 95% of commensal gut organisms are anaerobic and are difficult to recover by traditional (aerobic) culture techniques. Polymerase Chain Reaction (PCR) methodology identifies bacterial populations and is considered the standard for anaerobic bacteria assessment.
  • GI Effects assesses a set of 24 genera/species that map to 7 major phyla.

• Bacterial and mycology cultures demonstrate the presence of specific beneficial and pathological organisms.
• Bacterial and mycology sensitivities are provided for pathogenic or potentially pathogenic cultured organisms. The report includes effective prescriptive and natural agents.

• Parasitology includes comprehensive testing for all parasites on every parasitology exam ordered.
  • GI Effects provides microscopic fecal specimen examination for ova and parasites (O&P), the gold standard of diagnosis for many parasites.
  • 6 Polymerase chain reaction (PCR) targets detect common protozoan parasites including Blastocystis spp. with reflex subtyping 1-9, Cryptosporidium spp., Cyclospora cayetanensis, Dientamoeba fragilis, Entamoeba histolytica, and Giardia. PCR for organisms is emerging as a highly sensitive method for infectious organism detection.
  • Selection of a one-day or three-day sample collection is based on the clinician's clinical index of suspicion for parasitic infection. If there is no/low suspicion, a one-day sample will likely be adequate. For high suspicion, a three-day sample collection is optimal.

• Additional Biomarkers Available:
  • Campylobacter
  • Clostridium difficile
  • Escherichia coli
  • Helicobacter pylori
  • Macro Exam for Worms
  • KOH Preparation for Yeast
  • Zonulin Family Peptide
  • Fecal Lactoferrin

What Advantage Does the Microbial Ecology Profile Offer Compared to Other Diagnostics?

GI Effects® represents the best technical available to assess the gut microbiome, combining:

• 16S rRNA gene polymerase chain reaction (PCR) amplification technique for anaerobic commensal bacteria
• Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) technology for bacterial and fungal species identification via culture
• Gold standard microscopic ova and parasite (O&P) detection
• Real-time PCR for the identification of 6 common parasites
• Next-Generation DNA sequencing for Blastocystis spp. with reflex subtyping 1-9

The test report is organised so that the clinician can move through results in a logical order that enhances clinical utility, starting with the innovative Interpretation-At-A-Glance pages to synthesise the information.

What Can Clinicians and Patients Expect from GI Effects Microbial Ecology Profile Stool Testing?

The GI Effects Microbial Ecology Profile results provide insight into the root cause(s) of gut symptoms originating from a disturbed gut microbiome. Symptoms often improve as identified imbalances become normalised through targeted antimicrobial, dietary, lifestyle, and supplementation therapeutics.

The GI Effects Interpretive Guide is a convenient tool to support the therapeutic decision-making process for patients with complex gut-related conditions.

References

1. Marchesi J, et. al. The gut microbiota and host health: a new clinical frontier. Gut. 2016 Feb;65(2):330-9.
2. Clemente J, et. al. The impact of the gut microbiota on human health: an integrative review. Cell. 2012 Mar;148(6):1258-70.